Directional cloning

From WebRef.org
Revision as of 12:44, 27 September 2019 by Webref (talk | contribs) (Created page with "DNA insert and vector molecules are digested with two different restriction enzymes to create noncomplementary sticky ends at either end of each restriction fragment. This all...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

DNA insert and vector molecules are digested with two different restriction enzymes to create noncomplementary sticky ends at either end of each restriction fragment. This allows the insert to be ligated to the vector in a specific orientation and prevents the vector from recircularizing.


Sponsor: Download ANSI/ASQ Z1.4 Sampling Procedures Standard