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Reaction conditions--notably temperature, salt, and pH--that dictate the annealing of single-stranded DNA/DNA, DNA/RNA, and RNA/RNA hybrids. At high stringency, duplexes form only between strands with perfect one-to-one complementarity; lower stringency allows annealing between strands with some degree of mismatch between bases. | Reaction conditions--notably temperature, salt, and pH--that dictate the annealing of single-stranded DNA/DNA, DNA/RNA, and RNA/RNA hybrids. At high stringency, duplexes form only between strands with perfect one-to-one complementarity; lower stringency allows annealing between strands with some degree of mismatch between bases. | ||
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| + | [[Category: Biotechnology]] | ||
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Latest revision as of 14:50, 2 October 2019
Reaction conditions--notably temperature, salt, and pH--that dictate the annealing of single-stranded DNA/DNA, DNA/RNA, and RNA/RNA hybrids. At high stringency, duplexes form only between strands with perfect one-to-one complementarity; lower stringency allows annealing between strands with some degree of mismatch between bases.